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论文足球外围：Kang Wang, a,† Meng Zhang, b,† Yanna Huang, a Zhuolin Yang, Sha Su a and Maohua Chen a* .西北农林科技大学 植物保护学院 Characterisation of imidacloprid resistance in the bird cherry-oat aphid, Rhopalosiphum padi, a serious pest on wheat crops. Pest Management Science(2018)74(6):1457-1465.
论文摘要：BACKGROUND: Rhopalosiphum padi is a destructive insect pest of wheat worldwide. Studies have shown that R. padi has developed resistance to different insecticides, including imidacloprid. We studied the mechanisms conferring resistance to imidacloprid at the biochemical and molecular levels.
RESULTS: An R. padi imidacloprid-resistant (IM-R) strain and a susceptible (SS) strain were established. Fitness analysis using life-tables showed that the IM-R strain had obvious disadvantages in several parameters, indicating reduced fitness. Profiles of cross-resistance of IM-R and SS to seven insecticides were detected. Both synergistic and enzyme activity data suggested that P450 plays a role in resistance. Furthermore, the mRNA expression levels of cytochrome P450 (CYP) genes CYP6CY3-1 and CYP6CY3-2 were significantly increased in the IM-R strain. No target-site mutation within the nicotinic acetylcholine receptor (nAChR) subunits was detected in the IM-R strain. Interestingly, the expression levels of the nAChR 𝜶1, 𝜶2, 𝜶3, 𝜶7-2, and 𝜷1 subunit genes were significantly decreased, suggesting that down-regulation of these subunits may be involved in resistance.